Somatic Cell Nuclear Transfer
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Edited By:Peter SutovskyUniversity of Missouri-Columbia Columbia, Missouri, U.S.A. ISBN: 978-0-387-37753-7 Published: 2006-11-17 This book may be purchased as an eBook (pdf) for $99, or individual chapters (pdf) may be purchased from the list below for $19. |
Topics discussed include recent progress in pig SCNT and production of transgenic and knock out pigs for biomedical research and xenotransplantation, cattle cloning, behavioral studies in cloned mice, nuclear remodeling and imprinting, cell cycle regulation, nucleolar biogenesis, the effect of artificial activation on early development of cloned embryos, and inheritance of mitochondrial DNA and centrosome. Altogether, the present tome provides the reader with a unique developmental, molecular and cell biological view of the exciting SCNT technology, while also discussing its pitfalls and possible future directions and detours. Somatic Cell Nuclear Transfer will help both students and experienced researchers to further develop their knowledge and understanding of mammalian embryonic development.
Chapters available from this book
Nuclear Remodeling and Nuclear Reprogramming for Making Transgenic Pigs by Nuclear Transfer
Randall S. Prather
A better understanding of the cellular and molecular events that occur when a nucleus is transferred to the cytoplasm of an oocyte will permit the development of improved procedures for performing nuclear transfer and cloning. In some cases it appears that the gene(s) are reprogrammed, while i...
Somatic Cell Nuclei in Cloning: Strangers Traveling in a Foreign Land
Keith E. Latham, Shaorong Gao and Zhiming Han
The recent successes in producing cloned offspring by somatic cell nuclear transfer are nothing short of remarkable. This process requires the somatic cell chromatin to substi- tute functionally for both the egg and the sperm genomes, and indeed the processing of the transferred nuclei shares a...
Cloning Cattle: The Methods in the Madness
Björn Oback and David N. Wells
Somatic cell nuclear transfer (SCNT) is much more widely and efficiently practiced in cattle than in any other species, making this arguably the most important mammal cloned to date. While the initial objective behind cattle cloning was commercially driven—in particular to multiply genetically...
Centrosome Inheritance after Fertilization and Nuclear Transfer in Mammals
Qing-Yuan Sun and Heide Schatten
Centrosomes, the main microtubule organizing centers in a cell, are nonmembrane-bound semi-conservative organelles consisting of numerous centrosome proteins that typi- cally surround a pair of perpendicularly oriented cylindrical centrioles. Centrosome matrix is therefore oftentimes referred t...
Developmental, Behavioral and Physiological Phenotype of Cloned Mice
Kellie L. K. Tamashiro, Randall R. Sakai, Yukiko Yamazaki, Teruhiko Wakayama and Ryuzo Yanagimachi
Cloning from adult somatic cells has been successful in at least ten species. Although generating viable cloned mammals from adult cells is technically feasible, prenatal and perinatal mortality is high and live cloned offspring have had health problems. This chapter summarizes the health cons...
Nucleolar Remodeling in Nuclear Transfer Embryos
Jozef Laurincik and Poul Maddox-Hyttel
Transcription of the ribosomal RNA (rRNA) genes occurs in the nucleolus and results in ribosome biogenesis. The rRNA gene activation and the associated nucleolus forma- tion may be used as a marker for the activation of the embryonic genome in mammalian embryos and, thus serve to evaluate the d...
Somatic Cell Nuclear Transfer (SCNT) in Mammals: The Cytoplast and Its Reprogramming Activities
Josef Fulka, Jr. and Helena Fulka
It is now more than nine years since Dolly, the world’s first somatic cell cloned mammal was born, and the success of somatic cell nuclear transfer (SCNT) is still disappointingly low. Only about 3-5% of reconstructed embryos develop to term, and it is also evident that even if some clones are...
Mitochondrial DNA Inheritance after SCNT
Stefan Hiendleder
Mitochondrial biogenesis and function is under dual genetic control and requires extensive interaction between biparentally inherited nuclear genes and maternally inherited mitochondrial genes. Standard SCNT procedures deprive an oocytes’ mitochondrial DNA (mtDNA) of the corresponding maternal...
Activation of Fertilized and Nuclear Transfer Eggs
Christopher Malcuit and Rafael A. Fissore
In all animal species, initiation of embryonic development occurs shortly after the joining together of the gametes from each of the sexes. The first of these steps, referred to as “egg activation”, is a series of molecular events that results in the syngamy of the two haploid genomes and the ...
