Protein-Based Inheritance
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Edited By:Yury O. ChernoffGeorgia Institute of Technology ISBN: 978-1-58706-138-7 Published: 2007-09-04 This book may be purchased as an eBook (pdf) for $99, or individual chapters (pdf) may be purchased from the list below for $19. |
This book covers a topic that has been neglected for years and has returned to the spotlight only recently. Until the genetic role of DNA was firmly established, many researchers suspected that proteins, rather than nucleic acids, could be carriers of heritable information. However, these models were completely forgotten with the triumphal march of the double helix and the development of a central dogma postulating that information flow occurs strictly from DNA, through RNA, to protein, making it seemingly impossible for the proteins to possess a coding potential. Proteins were downgraded to the role of simple perpetuators and executors of DNA orders. Taken together, data included in this book prove beyond a reasonable doubt that proteins and multiprotein complexes are able to control heritable traits, and that, at least in some examples, this control occurs in a template-like fashion, so that new structures strictly reproduce patterns of pre-existing structures that were not specifically coded in DNA. Thus, protein-based inheritance has left the area of speculation and has emerged as a new topic amenable to high-quality experimental analysis.
Chapters available from this book
Yeast Prions: Evolution of the Prion Concept
Reed B. Wickner, Herman K. Edskes, Frank Shewmaker, Toru Nakayashiki, Abbi Engel, Linsay McCann and Dmitry Kryndushkin
Prions (infectious proteins) analogous to the scrapie agent have been identified in Saccharomyces cerevisiae and Podospora anserina based on their special genetic character istics. Each is a protein acting as a gene, much like nucleic acids have been shown to act as enzymes. The [URE3], [PSI+],...
A Short History of Small s: A Prion of the Fungus Podospora Anserina
Sven J. Saupe
Prions are infectious proteins. In fungi, prions correspond to non-Mendelian genetic elements whose mode of inheritance has long eluded explanation. The [Het-s] cytoplasmic genetic element of the filamentous fungus Podospora anserina, was originally identified in 1952 and recognized as a prion...
Prion and Nonprion Amyloids: A Comparison Inspired by the Yeast Sup35 Protein
Vitaly V. Kushnirov, Aleksandra B. Vishnevskaya, Ilya M. Alexandrov and Michael D. Ter-Avanesyan
Yeast prion determinants are related to polymerization of some proteins into amyloid-like fibers. The [PSI+] determinant reflects polymerization of the Sup35 protein. Fragmen- tation of prion polymers by the Hsp104 chaperone represents a key step of the prion replication cycle. The frequency of...
Biological Roles of Prion Domains
Sergey G. Inge-Vechtomov, Galina A. Zhouravleva and Yury O. Chernoff
In vivo amyloid formation is a widespread phenomenon in eukaryotes. Self-perpetuating amyloids provide a basis for the infectious or heritable protein isoforms (prions). At least for some proteins, amyloid-forming potential is conserved in evolution despite divergence of the amino acid (aa) se...
Preformed Cell Structure and Cell Heredity
Janine Beisson
This chapter will first recall the phenomena of “cortical inheritance” observed and genetically demonstrated in Paramecium 40 years ago, and later in other ciliates (Tetrahymena, Oxytricha, Paraurostyla), and will analyze the deduced concept of “cytotaxis” or “structural memory”. The significa...
Chaperone Effects on Prion and Nonprion Aggregates
Eugene G. Rikhvanov, Nina V. Romanova and Yury O. Chernoff
Exposure to high temperature or other stresses induces a synthesis of heat shock proteins. Many of these proteins are molecular chaperones, and some of them help cells to cope with heat-induced denaturation and aggregation of other proteins. In the last decade, chaperones have received increas...
Prion Stability
Brian S. Cox, Lee Byrne and Mick F. Tuite
The rate of spontaneous change from y- to the y+ condition determined in yeast by states of the Sup35p protein is briefly discussed, together with the conditions necessary for such change to occur. Conditions that promote and which affect the rate of induction of y+ in Sup35p and of other prio...
Prion-Prion Interactions
Irina L. Derkatch and Susan W. Liebman
The term prion has been used to describe self-replicating protein conformations that can convert other protein molecules of the same primary structure into its prion conformation. Several different proteins have now been found to exist as prions in Saccharomyces cerevisiae. Surprisingly, these...
The Genetic Control of the Formation and Propagation of the [PSI+] Prion of Yeast
Mick F. Tuite and Brian S. Cox
It is over 40 years since it was first reported that the yeast Saccahromyces cerevisiae contains two unusual cytoplasmic ‘genetic’ elements: [PSI+] and [URE3]. Remarkably the underlying determinants are protein-based rather than nucleic acid-based, i.e., that they are prions, and we have alrea...
Centriole Inheritance
Patricia G. Wilson
Early cell biologists perceived centrosomes to be permanent cellular structures. Centrosomes were observed to reproduce once each cycle and to orchestrate assembly of a transient mitotic apparatus that segregated chromosomes and a centrosome to each daughter at the completion of cell division....
