Chapter category: DNA
Dynamic Interaction between PARP-1, PCNA and p21waf1/cip1
Poly(ADP-Ribosyl)ation
Edited by: Alexander BurkleISBN: 0-387-33371-1
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Chapter authors:
Ennio Prosperi and A. Ivana Scovassi
Poly(ADP-ribose) polymerase (PARP-1) plays a crucial role in DNA repair and interacts with many DNA replication/repair factors, including the Proliferating Cell Nuclear Antigen (PCNA), a protein involved in many DNA transactions. The association between these proteins in vitro results in the inhibition of PARP-1 activity and of PCNA-dependent DNA synthesis carried out by DNA polymerase . In vivo, the interaction of the two proteins has been shown to increase after DNA damage. The activity of PCNA is regulated by p21waf1/cip1, which binds the interdomain connector loop of PCNA in the same region involved in the interaction of PCNA with DNA polymerase . This feature enables p21 to compete with DNA polymerase to regulate the differential inhibition of DNA replication vs. DNA repair. The finding that PARP-1 is associated with both PCNA and p21 suggests a possible cooperation of PARP-1 and p21 in regulating the functions of PCNA during DNA replication/repair.
Additional chapters from this book:
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