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Chapter category: Gene Expression

Reversal of Terminally Differentiated State in Skeletal Myocytes by SV40 Large T Antigen

This chapter appears in the following book:

Reactivation of the Cell Cycle in Terminally Differentiated Cells

Edited by: Marco Crescenzi
ISBN: 0-306-47423-9
» Get more information about this book at landesbioscience.com «

Chapter authors:
Takeshi Endo


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Terminal differentiation of mammalian skeletal muscle cells had long been thought to result in irreversible arrest in G0 phase of the cell cycle. Such terminally differentiated myotubes are refractory to apoptosis. However, I show here, using the SV40 large T antigen–inducible skeletal muscle cell line, that myotubes remain able to resume DNA synthesis, and undergo mitosis and cytokinesis. In addition, a subset of the T antigen–expressing myotubes die by apoptosis. The induction of c–Jun and Cdk_cyclin and downregulation of Rb and MyoD family proteins brought about by T antigen expression seem to be responsible for the reinduction of the cell cycle and apoptosis.

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Additional chapters from this book:

Reversal of Terminally Differentiated State in Skeletal Myocytes by SV40 Large T Antigen

Takeshi Endo

Terminal differentiation of mammalian skeletal muscle cells had long been thought to result in irreversible arrest in G0 phase of the cell cycle. Such terminally differe...

Cellular Dedifferentiation During Regeneration: The Amphibian Muscle System

Elly Tanaka

Amphibian limb regeneration represents a striking system where the reversal of muscle cell differentiation occurs in response to physiological stimuli. During this process, dedi...

Cell Cycle Reactivation in Skeletal Muscle and Other Terminally Differentiated Cells

Alessandra Sacco, Deborah Pajalunga, Lucia Latella, Francesca Siepi, Alessandro Rufini and Marco Crescenzi

This Chapter reviews, in a historical perspective, our current understanding of the cell cycle control in terminally differentiated skeletal muscle cells. Attempts at inducing reen...

Regulation of Cardiomyocyte Proliferation and Apoptosis

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Myocardial function is compromised in several forms of heart disease due to the loss of cardiomyocytes and in part to the limited ability of surviving myocytes to re–enter the...

Myocyte Proliferation in Heart Failure

Jan Kajstura, Annarosa Leri, Antonio Beltrami, Carlo A. Beltrami, Edmund H. Sonnenblick and Piero Anversa

The results summarized in this short Chapter challenge the perennial dogma that cardiac myocytes are terminally differentiated cells. Unequivocal evidence of mitosis is provided...


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