Chapter category: DNA
Identifying Clinicopathological Association of DNA Hypermethylation in Cancers Using CpG Island Microarrays
DNA Methylation and Cancer Therapy
Edited by: Moshe SzyfISBN: 0-306-47848-X
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Chapter authors:
Susan H. Wei, Timothy T.-C. Yip, Chuan-Mu Chen and Tim H.-M. Huang
Hypermethylation of promoter CpG islands has been associated with gene silencing in cancer. Increasingly, these CpG islands have potential clinical utility as molecular markers for cancer diagnosis. Here we describe a microarray-based technique, called differential methylation hybridization (DMH), for simultaneous screening of methylation alteration across thousands of CpG island loci in one tumor sample at a time. We also describe a second approach, called methylation target array (MTA), for detecting methylation alteration of a single CpG island locus across hundreds of tumor DNA samples. The DMH and MTA assays are complementary to each other in that DMH allows for rapid identification of multiple loci hypermethylated in tumor genomes while MTA can rapidly assess the utility of these loci as markers for clinical diagnosis. Furthermore, the use of clustering algorithms to analyze the array data of multiple CpG island loci can identify an association of DNA hypermethylation with specific clinicopathological features of tumors.
Additional chapters from this book:
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Identifying Clinicopathological Association of DNA Hypermethylation in Cancers Using CpG Island Microarrays
Susan H. Wei, Timothy T.-C. Yip, Chuan-Mu Chen and Tim H.-M. Huang
Hypermethylation of promoter CpG islands has been associated with gene silencing in cancer. Increasingly, these CpG islands have potential clinical utility as molecular markers for cancer diagnosis....
The Loss of Methyl Groups in DNA of Tumor Cells and Tissues: The Immunochemical Approach
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The existence of 5-methyldeoxycytidine (5-MeCyd) has been first demonstrated in 1958.1 For several years the presence of this naturally modified base in DNA remained unexplained and its role was ign...
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