Chapter category: Cell Metabolism
Nuclear Import of Agrobacterium T-DNA
Therapeutic Applications of Cell Microencapsulation
Edited by: Jose Luis Pedraz and Gorka OriveISBN: TBA
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Chapter authors:
Tzvi Tzfira, Benoit Lacroix and Vitaly Citovsky
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Additional chapters from this book:
The Molecular Mechanisms of mRNA Export
Tetsuya Taura, Mikiko C. Siomi and Haruhiko Siomi
A general paradigm for nuclear transport was established primarily through studies of protein import and export. Until recently, this paradigm was generally presumed also to apply to the process of ...
Nuclear Import and Export Signals
Toshihiro Sekimoto, Jun Katahira and Yoshihiro Yoneda
Eukaryotic cells are separated into two large compartments, namely the nucleus and the cytoplasm, by the nuclear envelope. As a result, macromolecules including RNAs, which are transcribed in the nu...
Structure of the Nuclear Pore
Michael Elbaum
The nucleus is a defining hallmark in cells of all the higher organisms: yeast, animals, and plants. As the repository of the genome, it both encloses the chromatin and regulates its accessibility. ...
Nuclear Export: Shuttling across the Nuclear Pore
John A. Hanover and Dona C. Love
One of the distinguishing features of eukaryotic cells is the compartmentalization of genetic information within a membrane-enclosed nucleus. The double membrane of the nuclear envelope separates th...
Nuclear Import of Agrobacterium T-DNA
Tzvi Tzfira, Benoit Lacroix and Vitaly Citovsky
Agrobacterium-mediated genetic transformation is a process by which genetic material is transported from the bacterium into the host nucleus, where it stably integrates. The transferred DNA (T-DNA) ...
Nuclear Import of DNA
David A. Dean and Kerimi E. Gokay
Nuclear trafficking of macromolecules usually brings to mind the nuclear import of NLS-containing proteins and certain RNAs and the export of NES-containing proteins and mRNAs. One macromolecule...
Integral Proteins of the Nuclear Pore Membrane
Merav Cohen, Katherine L. Wilson and Yosef Gruenbaum
The nuclear envelope contains three distinct membrane domains. The outer nuclear membrane faces the cytoplasm and is continuous with the rough endoplasmic reticulum (ER). Like the rough ER, th...
Nuclear Transport Methods
Jose Omar Bustamante
It is impossible to detail all of the techniques used in the nuclear transport field within the allotted space. Since some methods (EM, cell and molecular biology, etc.) are dealt with in othe...
Nuclear Import and Export of Mammalian Viruses
Michael Bukrinsky
Viruses are intracellular parasites that commandeer cellular processes, such as RNA processing or protein synthesis, to perform virus-specific functions. For this purpose, many viral protei...
Nuclear Protein Import: Distinct Intracellular Receptors for Different Types of Import Substrates
David A. Jans and Jade K. Forwood
Entry into the eukaryotic cell nucleus occurs through multiple pathways involving specific targeting signals, and intracellular receptor molecules of the importin/karyopherin superfamily which...
Regulation of Nuclear Import and Export of Proteins in Plants and its Role in Light Signal Transduction
Stefan Kircher, Thomas Merkle, Eberhard Schäfer and Ferenc Nagy
The nuclear envelope separates the theatres of two major cellular processes in eukaryotes: transcription takes place in the nucleus whereas proteins are synthesized in the cytoplasm. The lo...
Nuclear Import of Plant Proteins
Glenn R. Hicks
In this Chapter, we will focus primarily on protein import into the nucleus of plants. As in other eukaryotes the partitioning of genetic information into the nucleus necessitates the impor...
Subnuclear Trafficking and the Nuclear Matrix
Iris Meier
The nuclear matrix is the nuclear substructure that remains after the majority of DNA and soluble and chromatin-bound proteins have been removed from the nucleus. 1-3 Electron mi...
Epo Delivery by Genetically Engineered C2C12 Myoblasts Immobilized in Microcapsules
Ainhoa Murua, Gorka Orive, Rosa Ma Hernández and José Luis Pedraz
Over the last half century, the use of erythropoietin (Epo) in the management of malignancies has been extensively studied. Originally viewed as the renal hormone responsible for red blood cell produc...
Development of Subsieve-Size Capsules and Application to Cell Therapy
Shinji Sakai and Koei Kawakami
Reduction in the diameter of cell‑enclosing capsules has a practical application in cell therapy as it induces beneficial effects such as higher molecular exchangeability between the enclosed ce...
Regulatory Considerations in Application of Encapsulated Cell Therapies
J. van Zanten and P. de Vos
The encapsulation of tissue in semi‑permeable membranes is a technology with high potential and in due time several new therapies based on this technology will be tested in clinical trials. Rece...
