Chapter category: Viruses
Bacteriophage Lambda Terminase and the Mechanism of Viral DNA Packaging
Viral Genome Packaging Machines: Genetics, Structure
and Mechanism
Edited by: Carlos CatalanoISBN: 0-306-48227-4
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Chapter authors:
Michael Feiss and Carlos Enrique Catalano
The developmental pathways of many double-stranded DNA (dsDNA) viruses, both prokaryotic and eukaryotic, are remarkably similar. In viruses as diverse as bacteriophage λ and the herpesviruses, DNA replication proceeds through a rolling circle mechanism where the circular genome serves as a template for the synthesis of linear concatemers multiple genomes in length. Concurrently, viral gene expression produces structural proteins, which self-assemble into procapsids and, in the case of the bacteriophage, tails necessary to assemble an infectious virion. Virus assembly requires that monomeric virion DNA molecules be produced from concatemers during packaging of the DNA into a procapsid. Thus, packaging represents the convergence of the DNA replication and capsid shell assembly pathways. Genome packaging in bacteriophage λ has been extensively studied and this system has been used as a paradigm for virus assembly. Here we summarize current knowledge, present a working model, and indicate issues worthy of further investigation.
Additional chapters from this book:
Bacteriophage SPP1 DNA Packaging
Anja Droge and Paulo Tavares
SPP1 is a virulent double-stranded DNA (dsDNA) phage that infects the Gram-positive bacterium Bacillus subtilis strain 168. SPP1 belongs to the Siphoviridae family. The virion is composed of an icos...
DNA Packaging in Bacteriophage T4
Venigalla B. Rao and Lindsay W. Black
Double-stranded (ds) DNA packaging in phage T4 and other icosahedral viruses is a fascinating biological problem. During packaging, a complex, metabolically active, concatemeric DNA is translocated ...
Bacteriophage Lambda Terminase and the Mechanism of Viral DNA Packaging
Michael Feiss and Carlos Enrique Catalano
The developmental pathways of many double-stranded DNA (dsDNA) viruses, both prokaryotic and eukaryotic, are remarkably similar. In viruses as diverse as bacteriophage λ and the herpesviruses, ...
The o29 DNA Packaging Motor: Seeking the Mechanism
Dwight Anderson and Shelley Grimes
The Bacillus subtilis bacteriophage o29 research team in Minneapolis has marveled at (and reveled in) the intricacies of o29 assembly for more than 30 years. Here we highlight the current state of k...
Bacteriophage SPP1 DNA Packaging
Anja Dr?ge and Paulo Tavares
SPP1 is a virulent double-stranded DNA (dsDNA) phage that infects the Gram-positive bacterium Bacillus subtilis strain 168. SPP1 belongs to the Siphoviridae family. The virion is composed of an icos...
DNA Packaging by Bacteriophage P22
Sherwood Casjens and Peter Weigele
Bacteriophage P22 was isolated by Zinder and Lederberg1 a half century ago, and was immediately put to work by Salmonella bacterial geneticists because of its unusual (at that time) DNA packaging pr...
Cleavage and Packaging of Herpes Simplex Virus 1 DNA Herpesvirus Assembly
Joel D. Baines and Sandra K. Weller
Herpes simplex virus DNA accumulates in the nuclei of infected cells as long concatemers. The packaging machinery recognizes signals within the concatemers, cleaves the DNA to generate unit length m...
Encapsidation of the Segmented Double-Stranded RNA Genome of Bacteriophage PHI 6
Minna M. Poranen, Markus J. Pirttimaa and Dennis H. Bamford
Bacteriophage PHI6 has a segmented double-stranded RNA genome that is incorporated into a preformed capsid during the viral assembly. The three viral genomic segments are packaged as single-stranded...
T3/T7 DNA Packaging
Philip Serwer
During formation of a mature bacteriophage particle, a procapsid of protein packages the linear double-stranded DNA genome of the related bacteriophages, T3 and T7. Initiation of T3/T7 DNA packaging...
