Chapter category: Biotechnology
3xP3-EGFP Marker for Screening of Transgenic Silkworm
Transgenic Silkworms
Edited by: Katsutoshi YoshizatoISBN: 1-58706-176-7
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Chapter authors:
Jean-Luc Thomas
With the aim of Drosophila transgenesis the use of the transposable P element was the breakthrough for a new age of molecular genetic research in insects biology.1 The concern of experimentators was to identify the transgenic individuals quite easily as much as possible using vital screening markers. This was early possible for laboratory host strains such as Drosophila melanogaster using eye color mutants and their counterpart cloned genes able to rescue the color deficiency by transcomplementation. It was more difficult for other related dipteran species (nondrosophilid flies or mosquitoes) to benefit from such a prior genetic context. The situation was more critical in other insect orders comprising Lepidoptera, Hymenoptera or Orthoptera. It was particularly a slowing down in our attempts to transform Bombyx mori silkworm. I will present here a brief review based on some excellent already published reviews2-7 describing the importance of the availability of vital screening markers as a reliable detection for insect transgenesis technology. Constant progress on the quality and reliability of marker-coding sequences and more precisely the combination of those sequences with a convenient regulatory sequence has to be continued and encouraged. For Bombyx mori, we benefited by such progress as I will explain it in that chapter. Moreover I also consider that new progress could be envisaged using germ cells screening markers allowing very early in situ detection of transformed germ cells. We have also to keep in mind that the discovered fluorescent screening markers are really powerful essentially due to the progress made in the discovery and development of the new insect transposon as gene vectors.
Additional chapters from this book:
Transgenesis of the Silkworm by the Attenuated Nucleopolyhedrovirus
Hajime Mori, Masafumi Yamao and Masafumi Yamamoto
Nucleopolyhedrovirus (NPV) is a large, enveloped virus with a genome composed of covalently closed, double-stranded DNA and belongs to the family Baculoviridae.1 This virus has been developed into...
3xP3-EGFP Marker for Screening of Transgenic Silkworm
Jean-Luc Thomas
With the aim of Drosophila transgenesis the use of the transposable P element was the breakthrough for a new age of molecular genetic research in insects biology.1 The concern of experimentators w...
Transgenic Silkworms That Weave Recombinant Human Collagen into Cocoons
Masahiro Tomita, Katsuhiko Shimizu and Katsutoshi Yoshizato
The production of recombinant proteins on a large scale remains a major bottleneck in the biotechnology industry. Many recombinant expression systems have been developed using a variety of hosts such ...
Transgenic Analysis of the Biological Functions of a Doublesex Homologue in Bombyx mori
Masataka G. Suzuki and Toru Shimada
In D. melanogaster, a well-characterized cascade of genes, called the ‘sex determination cascade,’ is known to control somatic sex determination and differentiation.1-4 The primary genetic signal fo...
Use of piggyBac and a Sindbis Virus for Foreign Gene Expression and RNAi in the Silkworm
Marek Jindra and Mirka Uhlirova
Research on insects cannot advance without developing efficient methods for gene transfer, forced expression and silencing in vivo. Here we present recent developments of heat-inducible gene misexpr...
Preface
Jan Barciszewski, Volkmer Erdmann
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Noncoding RNAs Encoded by Bacterial Chromosomes
E. Gerhart H. Wagner and Jörg Vogel
Small noncoding RNAs are common to bacterial plasmids, phages and transposons, in which they regulate biological processes by acting as antisense RNAs. By contrast, only few small, noncoding R...
