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Chapter category: Development

Dissection of the Embryonic Brain Using Photoactivated Gene Expression

This chapter appears in the following book:

Brain Development
in Drosophila melanogaster

Edited by: Gerhard M. Technau
ISBN: 978-0-387-78260-7
» Get more information about this book at landesbioscience.com «

Chapter authors:
Jonathan Minden


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The Drosophila brain is generated by a complex series of morphogenetic movements. To better understand brain development and to provide a guide for experimental manipulation of brain progenitors, we created a fate map using photoactivated gene expression to mark cells originating within specific mitotic domains and time‑lapse microscopy to dynamically monitor their progeny. We show that mitotic domains 1, 5, 9, 20 and B give rise to discrete cell populations within specific regions of the brain. Mitotic domains 1, 5 , 9 and 20 give rise to brain neurons; mitotic domain B produced glial cells. Mitotic domains 5 and 9 produce the antennal and visual sensory systems, respectively, where each sensory system is composed of several disparate cell clusters. Time‑lapse analysis of marked cells showed complex mitotic and migratory patterns for cells derived from these mitotic domains.

Jonathan Minden
Department of Biological Sciences and Science, Carnegie Mellon University

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