Chapter category: Biotechnology
Self‑Contained, Fully Integrated Biochips for Sample Preparation, PCR Amplification and DNA Microarray Analysis
Integrated Biochips for DNA Analysis
Edited by: Robin Hui Liu and Abraham P. LeeISBN: 978-0-387-76758-1
» Get more information about this book at landesbioscience.com «
Chapter authors:
Robin Hui Liu, Piotr Grodzinski, Jianing Yang and Ralf Lenigk
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Robin Hui Liu
Osmetech Molecular Diagnostics
Piotr Grodzinski
National Cancer Institute
Jianing Yang
Applied NanoBioscience Center, Arizona State University
Ralf Lenigk
Applied NanoBioscience Center, Arizona State University
Additional chapters from this book:
Integrated DNA Biochips: Past, Present and Future
Piotr Grodzinski, Robin Hui Liu and Abraham P. Lee
DNA biochip is becoming a widespread tool used in life science, drug screening and diagnostic applications due to its many benefits of miniaturization and integration. The term “DNA biochip” is used b...
Integrated Molecular Analyses of Biological Samples on a Bead‑Based Microarray Platform
Joanne M. Yeakley, Daniel A. Peiffer, Marina Bibikova, Tim McDaniel, Kevin L. Gunderson, Richard Shen, Bahram G. Kermani, Lixin Zhou, Eugene Chudin, Shawn C. Baker, Kenneth M. Kuhn, Frank Steemers, Mark Hansen, Michael Graige , Celeste McBride, Steven Barnard, Bob Kain, David Barker and Jian-Bing Fan
Molecular analyses of biological samples have traditionally been pursued in parallel, with those researchers studying genetic diversity having few technical approaches in common with those studying ge...
Integrated Microfluidic CustomArray™ Biochips for Gene Expression and Genotyping Analysis
Robin Hui Liu, Mike Lodes, H. Sho Fuji, David Danley, Andrew McShea and Andrew McShea
DNA microarray technology has become one of the most promising analytical tools in molecular biology. It has been widely used for studying mRNA levels and examining gene expression in biological sampl...
Integrating Sample Processing and Detection with Microchip Capillary Electrophoresis of DNA
Adam T. Woolley
DNA is the storage medium for inherited information in living systems and thus, techniques for nucleic acid analysis are of great importance. Integrated microchip instrumentation for rapid separation ...
Integrated Plastic Microfluidic Devices for Bacterial Detection
Z. Hugh Fan and Antonio J. Ricco
This chapter describes integrated plastic microfluidic devices designed and fabricated for bacterial detection and identification. The devices, made from poly(cyclic olefin), contain components for DN...
Self‑Contained, Fully Integrated Biochips for Sample Preparation, PCR Amplification and DNA Microarray Analysis
Robin Hui Liu, Piotr Grodzinski, Jianing Yang and Ralf Lenigk
Rapid developments in back‑end detection platforms (such as DNA microarrays, capillary electrophoresis, real‑time polymerase chain reaction and mass spectroscopy) for genetic analysis have...
PCR in Integrated Microfluidic Systems
Victor M. Ugaz
Miniaturized integrated DNA analysis systems offer the potential to provide unprecedented advances in cost and speed relative to current benchtop‑scale instrumentation by allowing rapid bioanaly...
Integrated Nucleic Acid Analysis in Parallel Matrix Architecture
Jong Wook Hong
With the advent of the postgenomic era, accurate and fast nucleic analyses are becoming more important with reasonable running costs. Nucleic acids carry precious information important for the underst...
Chip‑Based Genotyping by Mass Spectrometry
Kai Tang
DNA analysis by mass spectrometry has the advantage of being the most accurate method for genotyping. Since the mass of molecular ions is measured and each allele has its own intrinsic mass, this meth...
Analyzing DNA‑Protein Interactions on a Chip
Limin Lin and James P. Brody
Gene expression is regulated by multi‑protein complexes binding to short noncoding regions of genomic DNA, called cis‑regulatory elements. A long‑term goal of genomics is to identify...
Single Molecule DNA Detection
Tza-Huei Wang, Christopher M. Puleo and Hsin-Chih Yeh
The development of single‑molecule detection (SMD) technologies that allow measurements of intra and intermolecular interactions at the most fundamental level has greatly advanced basic research...
Nanochannels for Genomic DNA Analysis: The Long and the Short of It
Robert Riehn, Walter Reisner, Jonas O. Tegenfeldt, Yan Mei Wang, Chih-kuan Tung, Shuang-Fang Lim, Edward Cox, James C. Sturm, Keith Morton, Steven Y. Chou and Robert H. Austin
This review will discuss the theory of confined polymers in nanochannels and present our experiments, which test the theory and explore use of nanochannels for genomic analysis. Genomic length DNA mo...
Beyond Microtechnology—Nanotechnology in Molecular Diagnosis
Paolo Fortina, Joseph Wang, Saul Surrey, Jason Y. Park and Larry J. Kricka
Advances in technology have led to significant improvements in our ability to detect and diagnose disease. In particular, the advent of nucleic acid technology has allowed unparalleled insight into th...
