Adenoviral Gene Therapy for Malignant Pleural Mesothelioma

Daniel H. Sterman, Larry R. Kaiser, and Steven M. Albelda

Malignant mesothelioma is a primary neoplasm of the mesothelial lining of the pleural (80%) or peritoneal cavities (19.5%). It has been linked conclusively to prior exposure to ...

Opportunities for p53 Tumor Suppressor Gene Therapy in Ovarian and

Loretta L. Nielsen, Mark Pegram, Beth Karlan, John Elkas, and Jo Ann Horowitz

Schering–Plough Research Institute is currently sponsoring phase I/II clinical trials of adenovirus–mediated p53 gene therapy for cancer in several countries. The drug used in these ...

Adenoviral Vectors for Liver Cancer—Clinical Trials

Ragai R. Mitry, Catherine E. Sarraf, Nagy A. Habib

Malignant tumors of the liver are among the commonest cancers in the world, with an estimated annual global incidence of 1,000,000 cases per year.¹ This includes primary tumors such...

Ad& - p53 Clinical Trial in Patients with Squamous Cell Carcinoma

Gary L. Clayman, Douglas K. Frank, and Patricia A. Bruso

Our laboratory has been involved in the investigation of wild type p53 gene transfer for the selective induction of apoptosis in human upper aerodigestive tract squamous cell carcinoma of t...

AdCFTR for Cystic Fibrosis

Samuel C. Wadsworth

Cystic fibrosis (CF) is a recessive, monogenic, lethal disorder caused by defects in a protein termed the cystic fibrosis transmembrane conductance regulator (CFTR) .^1,2 Approxim...

Host Immune Responses to Recombinant Adenoviral Vectors

Johanne M. Kaplan

Recombinant adenoviruses (Ad) are versatile gene delivery vectors capable of infecting a broad range of cell types without a requirement for cell division. They are being developed for a va...

Implications of the Innate Immune System for Adenovirus-Mediated Gene Transfer

Kazuhisa Otake and Bruce C. Trapnell

Adenovirus is one of the most well-studied viruses, partly due to its use as a model of eukaryotic gene expression and partly due to efforts to develop a vaccine against outbreaks of adenov...

Adenovirus–Host Interactions to Subvert the Host Immune System

William S. M. Wold and Ann E. Tollefson

Viruses have evolved ingenious mechanisms to evade the host antiviral response. The first to be recognized is antigenic variation, which allows viruses to escape preexisting antibodies, and is...

Adenovirus-Induced Pathogenesis

Harold S. Ginsberg

With the discovery of antibiotics during World War II, the use of tissue culture soon became relatively easy after the war had ended. These findings led to the discovery of many new viruses...

Homologous Recombination Between Exogenous and Integrated Adenovirus

C. S. H. Young and Gregory J. Duigou

The recent dramatic increase in the use of adenovirus as a transduction vector has led to a renewed interest in all phases of its life cycle. Among the issues that have received increased atte...

Adenovirus Transformation and Tumorigenicity

Robert P. Ricciardi

In natural infection of humans, adenoviruses generally enter differentiated, quiescent epithelial cells. For successful propagation of virus to occur, these infected cells need to be growth st...

Adenoviral Vectors for the Manipulation of Human Hematolymphoid Cells: Purging and Other Applications

Timothy C. Meeker, Joanne M. Wroblewski and Prem Seth

The human bone marrow produces a complex array of specialized cells designed to function in a myriad of ways. The pluripotent hematopoietic stem cell (HSC) gives rise to committed progenito...

Development of an E1B, 55 kDa Gene–Deleted, Selectively Replicating

David H. Kirn

The vast majority of human cancers are incurable once metastatic. Chemotherapy and radiotherapy can induce tumor growth inhibition or regression in some cases, but solid tumor progression a...

Transcriptional and Promoter–Driven Control of Adenovirus–Mediated

Yoko Yoshida and Hirofumi Hamada

Transcriptionally targeted adenoviral vectors which are able to restrict and regulate the levels of expression of the therapeutic gene will have wide applicability for gene therapy. For cancer...

Adenovirus-AAV Combination Strategies for Gene Therapy

Krishna J. Fisher

Among the repertoire of eukaryotic viruses that have been genetically engineered for targeting therapeutic genes to somatic cells, adenovirus has captured considerable attention since the earl...

Strategies to Adapt Adenoviral Vectors for Gene Therapy Applications

Joanne T. Douglas, Meizhen Feng, and David T. Curiel

The Generation of Targeted Adenoviral Vectors by Immunological Modifications of the Fiber Protein

Full utility of adenoviral vectors is potentially undermined by their broad tropis...

Adenoviral Vectors for Vaccines

Bernard Klonjkowski, Caroline Denesvre, and Marc Eloit

Several viruses can be used for the development of vectors for vaccination purposes. The human adenovirus type 2 (Ad2) and 5 (Ad5) have been used most extensively because of the greater unders...

Correction of Serum Protein Deficiencies with Recombinant Adenoviral

James N. Higginbotham and Prem Seth

There are numerous inherited and acquired serum protein deficiencies that cause moderate to severe and even life threatening diseases in people. Some of the diseases include

Adenovirus-Mediated Gene Transfer: Applications in Lipoprotein Research

Silvia Santamarina-Fojo and Marcelo J.A. Amar

In the last decade adenovirus–mediated in vivo gene transfer has been effectively utilized to enhance our understanding of the role that different apolipoproteins, enzymes, recepto...

Adenovirus Vectors for Therapeutic Gene Transfer to Skeletal Muscles

Josephine Nalbantoglu, Basil J. Petrof, Rénald Gilbert, and George Karpati

Duchenne's muscular dystrophy (DMD) and the less severe Becker muscular dystrophy (BMD) result from mutations in the dystrophin gene on chromosome Xp21.¹ DMD, which afflicts approxi...

IAP–Based Gene Therapy for Neurodegenerative Disorders

Stephen J. Crocker, Daigen Xu, Charlie S.Thompson, Peter Liston, and George S. Robertson

Neurodegenerative diseases are steadily becoming more prominent as human lifeexpectancy increases. Gene therapy holds tremendous promise for the long term treatment of neurodegenerative disord...

Adenoviral Vectors for Cardiovascular Diseases

Noel M. Caplice, Timothy O'Brien, and Robert D. Simari

Cardiovascular diseases affect over 60 million people in the United States, and resulted in over 150 billion dollars in expenses in 1996 (American Heart Association, 1997). As such, the pot...

Adenoviral Vectors for Cancer Gene Therapy

Prem Seth, Yu Katayose, and Amol N.S. Rakkar

Cancer can be considered a "genetic" disorder, and thus a legitimate target for gene therapy, in as much as it arises as a result of a stepwise accumulation of genetic defects in a clone of...

Development of Adeniviral Vectors for Gene Therapy

Dai Katayose, Prem Seth

In recent years adenoviruses have been extensively used as vectors to deliver foreign genome into mammalian cells (reviewed in refs. 1-5). Adenoviruses have certain features, which make them a...

Adenovirus Assembly

Susanne I. Schmid and Patrick Hearing

The assembly of adenovirus particles proceeds through an ordered series of assembly events (reviewed in ref. 1). The assembly of the virus particles has been probed using viral temperature-con...

Role of Endoprotease in Adenovirus Infection

Joseph Weber

With the possible exception of very simple viruses, most viruses appear to encode at least one virus-specific endopeptidase. In addition to facilitating the orchestrated fragmentation of po...

Adenovirus Late Gene Expression

Julie Boyer and Gary Ketner

The late phase of an adenovirus infection begins with the onset of viral DNA replication. During the late phase, protein synthesis in adenovirus-infected cells is dominated by the production o...

Adenovirus DNA Replication

Muralidhara Ramachandra and R. Padmanabhan

After adenovirus (Ad) enters the cell by receptor–mediated endocytosis, the viral DNA is uncoated and transported to the nucleus. Beginning at 6–8 hours post–infection, DNA is e...

Early Gene Expression

Philip E. Branton

The major targets of human adenoviruses are terminally differentiated epithelial cells of the upper respiratory tract, gut and eye. Once the virus has entered the cell and its DNA uncoated app...

Entry of Adenovirus into Cells

Prem Seth

With the advent of the electron microscope, it became possible to study the entry of adenoviruses into eukaryotic cells. Time course experiments of adenovirus uptake into the cells indicated a pat...

Organization of the Adenoviral Genome

Jane Flint

The DNA genomes of adenoviruses have been the objects of intense scrutiny since the first representative of this virus family was isolated in the winter of 1953–54.¹ Both th...

Adenovirus Capsid Proteins

John J. Rux and Roger M. Burnett

Since Rowe et al¹ first isolated adenovirus from human adenoid cells, more than 100 different species have been identified from various mammals. The family is characterized by th...

Discovery and Classification of Adenoviruses

Harold S. Ginsberg

In 1953, Wallace Rowe was then a postdoctoral fellow at the National Institutes of Health with Robert Huebner; they were working, with their colleagues, to isolate the "virus of the common ...